2024 Megan Sheridan Western Blots

Published: 11 January 2024| Version 2 | DOI: 10.17632/zbppjnyyrr.2
Contributors:
Megan Sheridan, Besim Ogretmen

Description

Opportunistic anaerobic pathogen Porphyromonas gingivalis (P.gingivalis) has been associated with poor clinical outcomes in patients with oral squamous cell carcinomas (OSCC). However, precise roles and mechanisms by which P.gingivalis infection enhances OSCC growth/proliferation or resistance to cell death remain elusive. Here, we determined that P.gingivalis infection mediates therapeutic resistance via inhibiting lethal mitophagy in OSCC cells and orthotopic tumors.

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Steps to reproduce

UMSCC1A cells were infected with P. gingivalis (100 MOI) for 6 hours followed by treatment with vehicle or LCL768 (30 uM, 2 hours).

Institutions

Medical University of South Carolina

Categories

Western Blot

Funding

National Institute of Dental and Craniofacial Research

R01DE030313

National Institute of Dental and Craniofacial Research

T32DE017551

National Institutes of Health

AG069769, CA214461, CA214461-S1, DE016572, DE016572-S1, and P01 CA203628

Licence