Medical University of South Carolina Showcase

MS analysis of proteins from 1A cells identified using immunoprecipitation with 2SC antibody and silver stained. The samples are as follows: vehicle, Fumaric Acid (FA) (10mM), LCL768 (10 uM), and a combination of FA and LCL879.

Supplemental manuscript materials for JAAD manuscript "A Randomized Controlled Trial Analyzing Nonthermal Atmospheric Plasma for the Treatment of Verruca Vulgaris in Pediatric Patients". Supplemental Figure 1. Floating‐electrode dielectric barrier device plasma pulse generator and hand‐held electrode. The tip of the electrode connected to the pulse generator must be held near the treated surface. Supplemental Table 1. Demographic data of patients enrolled for treatment of verruca vulgaris with either nonthermal atmospheric plasma or standard of care therapy. Supplemental Table 2. Physician-determined and patient-reported post-treatment adverse events and sequelae following treatment of verruca vulgaris lesions with nonthermal atmospheric plasma or standard of care therapy.

Western blot and microscopy images of the article, A highly potent bi-thiazole inhibitor of LOX rewires collagen architecture and enhances chemoresponse in triple-negative breast cancer.

Supplemental images for JAAD Dermatology Images - Pityriasis Rubra Pilaris

Raw WB images. Programmed death-ligand 1, PD-L1 (CD274) facilitates immune evasion and exerts pro-survival functions in cancer cells. Here, we report a new mechanism whereby internalization of PD-L1 in response to alterations of bioactive lipid/ceramide metabolism by ceramide synthase 4 (CerS4) induces sonic-hedgehog (Shh) and TGF- receptor signaling to enhance tumor metastasis in triple-negative breast cancers (TNBC), exhibiting immunotherapy resistance. Mechanistically, data showed that internalized PD-L1 interacts with an RNA-binding protein Caprin-1 to stabilize Shh/TGFBR1/Wnt mRNAs to induce -catenin signaling and TNBC growth/metastasis, consistent with increased infiltration of FoxP3+ T regs and resistance to immunotherapy. While mammary tumors developed in MMTV-PyMT/CerS4-/- were highly metastatic, targeting the Shh/PD-L1 axis using Sonidegib and anti-PD-L1 antibody vastly decreased tumor growth and metastasis, consistent with the inhibition of PD-L1 internalization and Shh/Wnt signaling, restoring anti-tumor immune response. These data, validated in clinical samples and databases, provide a mechanism-based therapeutic strategy to improve immunotherapy responses in metastatic TNBCs.

Mechanisms by which aging Alzheimer’s disease (AD) patients exhibit decreased cancer incidence are mainly unknown. Here, we demonstrate that mitochondrial localization of AD-associated amyloid precursor protein (APP) and its cleaved amyloid-β 40 (Aβ40), but not the mutant APP that lacks mitochondrial localization signal, inhibits lipid stress-mediated hyperactive mitophagy in aging T-cells, improving their anti-tumor functions. B16-derived melanoma or carcinogen-induced oral cancer growth is highly reduced in 3xTg AD mice, which is attenuated by T-cell depletion. Adoptive cell transfer (ACT)-based immunotherapy using aging T-cells isolated from 3xTg-AD/Pmel mice controlled B16-melanoma tumor growth in recipient mice compared to controls. The metabolic signature of aging stress-dependent mitophagy in mouse and human T-cells showed depletion of fumarate, and exogenous fumarate supplementation or whole mitochondria transfer phenocopied aging AD T-cells with improved anti-tumor functions in aging WT T-cells. Thus, this study explains how AD is linked to improved anti-tumor immunity which limits aging-stress-dependent tumor growth.

Supplementary tables for: Pediatric Allergic Contact Dermatitis Registry Patch Testing Results from 2016-2022: A Retrospective Study of Age-Related Differences

Original, uncropped western blot image files.

Description of methods used to compile a comprehensive list of online educational dermatopathology resources.

We have compiled a comprehensive list of online dermatopathology resources that residents and fellows in training may utilize. Here is a list of interactive/static virtual slide libraries and virtual case studies, all of which are accessible free of charge.